Provided at OPENSCREEN-GR by
- NCSR “Demokritos” Athens
Services
Protein Production
Recombinant Protein Production - NCSR “Demokritos” Athens
IΒA offers facilities for recombinant protein production using bacterial, insect and mammalian expression systems and for protein purification.
Equipment
- Refrigerated incubators for protein expression in bacteria or insect cells
- CO2 incubators for protein expression in mammalian cells
- AKTA Purifier FPLC System
Characteristics
AKTAFPLC
- Flow-rate range:05–20 ml/min
- Pressure range: 0–5 MPa (50 bar, 725 psi)
- Wavelength selection: 254, 280, 313, 405, 436, 546 nm (with Hg lamp)
- Conductivity range: 1 μS/cm–999.9 mS/cm
- pH range: 0–14
- Solvent compatibility: All solvents and buffers commonly used in chromatography of biomolecules
- Additional sample pump
Applications
Produced recombinant proteins can be used in:
- screening small-molecules that affect enzymatic activities leading to change in color, fluorescence, pH
- screening of drug-protein binding
- screening small-molecules for protein conformational changes
