+30 210 650 3533 openscreen@bio.demokritos.gr

Provided at OPENSCREEN-GR by

  • NCSR “Demokritos” Athens


Protein Production

Recombinant Protein Production - NCSR “Demokritos” Athens

IΒA offers facilities for recombinant protein production using bacterial, insect and mammalian expression systems and for protein purification.


  • Refrigerated incubators for protein expression in bacteria or insect cells
  • CO2 incubators for protein expression in mammalian cells
  • AKTA Purifier FPLC System



  • Flow-rate range:05–20 ml/min
  • Pressure range: 0–5 MPa (50 bar, 725 psi)
  • Wavelength selection: 254, 280, 313, 405, 436, 546 nm (with Hg lamp)
  • Conductivity range: 1 μS/cm–999.9 mS/cm
  • pH range: 0–14
  • Solvent compatibility: All solvents and buffers commonly used in chromatography of biomolecules
  • Additional sample pump


Produced recombinant proteins can be used in:

  • screening small-molecules that affect enzymatic activities leading to change in color, fluorescence, pH
  • screening of drug-protein binding
  • screening small-molecules for protein conformational changes

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