Provided at OPENSCREEN-GR by
- NCSR “Demokritos” Athens
- Democritus University of Thrace
- University of Ioannina
Services
Multimode Microplate Reader - NCSR “Demokritos” Athens
Multi-parametric spectrophotometer suitable for rapid screening techniques by measuring absorption, fluorescence and chemilluminescence
Equipment
Tecan Spark™
Characteristics
- ELISA analysis
- Dispenser
- Temperature and humidity control
- Incubator (CO2 / O2 integrated units)
- Automated microscope
- Cytometer
- Absorption
- Fluorescence
- Luminescence
- High speed Monochromator, High speed Monochromator (HSM)
- Spectrum: 200nm – 1000nm
- DNA – RNA qualitative analysis analysis (206/280
Applications
- Quantification of nucleic acid (absorption)
- Quantification of nucleic acid (fluorescence)
- Cell fluorescence
- Luminescence measurement
- Cell incubation
- High-throughput screening up to 50×384 wells per measurement
- Imaging (cell imagining)
- Measurement (cell counting)
Multiplex & Robotics
Multiplex Analysis - NCSR “Demokritos” Athens
A technology based on microbead flow cytometry and analyzes hydrodynamic, optical and digital data allowing multiple simultaneous analysis of targets per sample or well.
Equipment
Luminex MagPix™
Characteristics
- Multiplexing: Up to 50 analyzers per sample
- Sensitivity: About 100 copies of DNA or pg protein
- Reading time: Plate 96 wells in ≤60 minutes
Applications
- Coupling protein
- Immunoassays
- Serology techniques
- Nucleic Acids
Robotic Microplate Handling System - NCSR “Demokritos” Athens
Equipment
Freedom EVO75™, TECAN
Characteristics
Freedom EVO75 robotic system undertakes the repetitive fluid handling required in a high-performance experimental protocol with large number of wells or with multiple compounds.
Applications
- Accurate dilutions
- Limiting dilutions
- Equal distributions
Flow Cytometry
Flow Cytometry - NCSR “Demokritos” Athens
Equipment
BD FACSCalibur™
Characteristics
- Two lasers
- Fluorescent detectors and filters
- Up to four high-performance, high-dynamic range amplifiers with filters: 530 nm (FITC), 585 nm (PE / PI), and> 670 nm (PerCP), and 661 nm (APC) – FL4 option
Applications
- Cell cycle analysis and detection of cell apoptosis
- Bacterial studies
- Extracellular markers
- Intracellular staining
Flow Cytometry - Democritus University of Thrace
The unit is equipped with Attune NxT acoustic flow cytometer (Thermo Fischer Scientific) and the Attune NxT Software. The flow cytometer has two lasers: 488 nm, 50 mW (blue) and 637 nm (100 mW) which allow the retrieval of up to nine different parameters, up to seven color fluorescence data plus front and side scattering. Acoustic focus technology allows the analysis of up to 35,000 events per second.
Equipment:
Attune NxT (Thermo Fischer Scientific)
Application
- Detection of molecular markers of cytotoxicity by multiple labeling using antibodies
- Cell cycle analysis and cell death studies (necrosis, apoptosis, necropsy, autophagy)
- Protein expression analysis
- Precision multimeter analyzes to assess the toxicity and biological activity of bioactive molecules
- Investigation of anti-inflammatory action
- Determination of oxidative damage, characterization of pre / anti-oxidant action
- Evaluation of antimicrobial activity
Flow Cytometry - University of Ioannina
Equipment
BD Accuri C6 Plus
Characteristics
- It has two lasers (488nm, and 640nm or respectively).
- Analyzes the following parameters: FSC, SSC and at least 4 fluorescences simultaneously in linear or logarithmic form.
- Detection limit: 0.5 μm Recommended effluent: filtered and distilled water
- The analyzer can also enter a threaded conductor if required. Dimensions should be mentioned.
Applications
- Bioactivity in cell samples
- Cell cycle analysis
- Expression of protein markers
